Isolation:Article Title: Pan-cancer proteogenomics expands the landscape of therapeutic targets.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-integrin beta 5 Proteintech Cat# 28543-1-AP; RRID: AB_2881167 Rabbit monoclonal anti-CAD (clone D2T8H) Cell Signaling Technology Cat# 93925; RRID: AB_2750933 Rabbit anti-PAK2 Cell Signaling Technology Cat# 2608; RRID: AB_2283388 Mouse anti-GAPDH-HRP Yeasen Cat# 30203ES10 Mouse anti-IFN-g (clone 1-D1K) Mabtech Cat# 3420-3-1000; RRID: AB_907282 Mouse anti-IFN-g, biotin-conjugated (clone 7-B61) Mabtech Cat# 3420-6-1000; RRID: AB907272 Goat anti-rabbit IgG (H+L) HRP Yeasen Cat# 33101ES60; RRID: AB2922405 Biological samples PBMCs isolated from peripheral blood in healthy donors This paper Zhongshan Hospital, Fudan University PBMCs isolated from peripheral blood in healthy donors This paper Baylor College of Medicine Chemicals, peptides, and recombinant proteins Peptides China Peptides Inc. Genemed Synthesis Inc. N/A RPMI-1640 media cytiva Cat# SH30096.01 Click’s media IrvineScientific Cat# 9195 Human Ab serum Valley Biomedical, Inc. Cat# HP1022 Glutamax gibco Cat# 35050-061 Lymphoprep StemCell Technologies Cat# 07851 ImmunoCult-XF T cell expansion medium StemCell Technologies Cat# 10981 ImmunoCult Human CD3/CD28 T cell activator StemCell Technologies Cat# 10971 CD14 magnetic microbeads Miltenyi Biotec Cat# 130-050-201 CellGenix GMP DC media CellGenix Cat# 20801-0500 IL-1b R&D Systems Cat# 201-LB-025 IL-2 (Teceleukin) NCBI Cat# Ro 23-6019 IL-4 R&D Systems Cat# 204-IL/CF-MTO IL-6 R&D Systems Cat# 206-IL-050/CF IL-7 R&D Systems Cat# BT-007-01M IL-12 InvivoGen Cat# rcyc-hil12 IL-15 R&D Systems Cat# BT-015-01M GM-CSF R&D Systems Cat# 215-GM/CF-MTO TNF-ɑ R&D Systems Cat# 210TA100 PGE-2 Sigma Cat# P6532-1MG PHA-L Sigma-Aldrich Cat# L4144 Avidin-peroxidase solution Vector Laboratories Cat# PK-6100 AEC Sigma-Aldrich Cat# A6926 Tumor-associated antigens GenScript Biotech Corporation ImmunoCult Human CD3/CD28 T Cell Activator Stemcell Cat# 10971 Human recombinant IL-2 Stemcell Cat# 78036 Alvespimycin Selleckchem Cat# S1142 Tanespimycin Selleckchem Cat# S1141 Lipofectamine 3000 Thermo Cat# L3000015 Cell Counting Kit (CCK-8) Yeasen Cat# 40203ES80 (Continued on next page) e1 Cell 187, 1–19.e1–e10, August 8, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER NCI-H1944 Yongbo Wang lab (Department of Cellular and Genetic Medicine, School of Basic Medical Sciences, Fudan University) RRID: CVCL_1508 HT29 Dawei Li lab (Department of Colorectal Surgery, Fudan University Shanghai Cancer Center) RRID: CVCL_0320 Ishikawa QuiCell Biotechnology RRID: CVCL_2529 LOVO Daming Gao lab (Shanghai Institute of Biochemistry and Cell Biology) RRID: CVCL_0399 BXPC3 Yi Qin lab (Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center) RRID: CVCL_0186 Experimental models: Organisms/strains BALB/c Nude Shanghai Model Organisms Center Cat# SM-014 Oligonucleotides See Table S5B This study N/A Software and algorithms PepQuery2 Wen and Zhang12 http://www.pepquery.org metap Dewey68 https://cran.r-project.org/web/packages/ metap/index.html WebGestalt Liao et al.69 https://www.webgestalt.org GISTIC2.0 Mermel et al.70 ftp://ftp.broadinstitute.org/pub/ GISTIC2.0/GISTIC_2_0_23.tar.gz ragp Dragi cevi c et al.71 https://github.com/missuse/ragp protr Xiao et al.72 https://cran.r-project.org/web/ packages/protr/ NeoFlow Wen et al.37 https://github.com/bzhanglab/neoflow ComplexHeatmap Gu et al.73 https://bioconductor.org/packages/ release/bioc/html/ComplexHeatmap.html Optitype Szolek et al.74 github.com/FRED-2/OptiType NetMHCpan Jurtz et al.75 https://services.healthtech.dtu.dk/ service.php?NetMHCpan-4.0 customprodbj Wang et al.76 https://github.com/bzhanglab/ customprodbj PDV Li et al.77 https://github.com/wenbostar/PDV InteractiVenn Heberle et al.78 http://www.interactivenn.net/ ZigZag Thompson et al.79 https://github.com/ammonthompson/ zigzag KSEA Casado et al.80 and Wiredja et al.81 https://cran.r-project.org/web/ packages/KSEAapp/index.html Cytoscape v3.10.0 Shannon et al.82 https://cytoscape.org/ drc v3.0-1 Ritz et al.83 https://cran.r-project.org/web/ packages/drc ll OPEN ACCESS Please cite this article in press as: Savage et al., Pan-cancer proteogenomics expands the landscape of therapeutic targets, Cell (2024), https://doi.org/10.1016/j.cell.2024.05.039 Resource
Recombinant:Article Title: Pan-cancer proteogenomics expands the landscape of therapeutic targets.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-integrin beta 5 Proteintech Cat# 28543-1-AP; RRID: AB_2881167 Rabbit monoclonal anti-CAD (clone D2T8H) Cell Signaling Technology Cat# 93925; RRID: AB_2750933 Rabbit anti-PAK2 Cell Signaling Technology Cat# 2608; RRID: AB_2283388 Mouse anti-GAPDH-HRP Yeasen Cat# 30203ES10 Mouse anti-IFN-g (clone 1-D1K) Mabtech Cat# 3420-3-1000; RRID: AB_907282 Mouse anti-IFN-g, biotin-conjugated (clone 7-B61) Mabtech Cat# 3420-6-1000; RRID: AB907272 Goat anti-rabbit IgG (H+L) HRP Yeasen Cat# 33101ES60; RRID: AB2922405 Biological samples PBMCs isolated from peripheral blood in healthy donors This paper Zhongshan Hospital, Fudan University PBMCs isolated from peripheral blood in healthy donors This paper Baylor College of Medicine Chemicals, peptides, and recombinant proteins Peptides China Peptides Inc. Genemed Synthesis Inc. N/A RPMI-1640 media cytiva Cat# SH30096.01 Click’s media IrvineScientific Cat# 9195 Human Ab serum Valley Biomedical, Inc. Cat# HP1022 Glutamax gibco Cat# 35050-061 Lymphoprep StemCell Technologies Cat# 07851 ImmunoCult-XF T cell expansion medium StemCell Technologies Cat# 10981 ImmunoCult Human CD3/CD28 T cell activator StemCell Technologies Cat# 10971 CD14 magnetic microbeads Miltenyi Biotec Cat# 130-050-201 CellGenix GMP DC media CellGenix Cat# 20801-0500 IL-1b R&D Systems Cat# 201-LB-025 IL-2 (Teceleukin) NCBI Cat# Ro 23-6019 IL-4 R&D Systems Cat# 204-IL/CF-MTO IL-6 R&D Systems Cat# 206-IL-050/CF IL-7 R&D Systems Cat# BT-007-01M IL-12 InvivoGen Cat# rcyc-hil12 IL-15 R&D Systems Cat# BT-015-01M GM-CSF R&D Systems Cat# 215-GM/CF-MTO TNF-ɑ R&D Systems Cat# 210TA100 PGE-2 Sigma Cat# P6532-1MG PHA-L Sigma-Aldrich Cat# L4144 Avidin-peroxidase solution Vector Laboratories Cat# PK-6100 AEC Sigma-Aldrich Cat# A6926 Tumor-associated antigens GenScript Biotech Corporation ImmunoCult Human CD3/CD28 T Cell Activator Stemcell Cat# 10971 Human recombinant IL-2 Stemcell Cat# 78036 Alvespimycin Selleckchem Cat# S1142 Tanespimycin Selleckchem Cat# S1141 Lipofectamine 3000 Thermo Cat# L3000015 Cell Counting Kit (CCK-8) Yeasen Cat# 40203ES80 (Continued on next page) e1 Cell 187, 1–19.e1–e10, August 8, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER NCI-H1944 Yongbo Wang lab (Department of Cellular and Genetic Medicine, School of Basic Medical Sciences, Fudan University) RRID: CVCL_1508 HT29 Dawei Li lab (Department of Colorectal Surgery, Fudan University Shanghai Cancer Center) RRID: CVCL_0320 Ishikawa QuiCell Biotechnology RRID: CVCL_2529 LOVO Daming Gao lab (Shanghai Institute of Biochemistry and Cell Biology) RRID: CVCL_0399 BXPC3 Yi Qin lab (Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center) RRID: CVCL_0186 Experimental models: Organisms/strains BALB/c Nude Shanghai Model Organisms Center Cat# SM-014 Oligonucleotides See Table S5B This study N/A Software and algorithms PepQuery2 Wen and Zhang12 http://www.pepquery.org metap Dewey68 https://cran.r-project.org/web/packages/ metap/index.html WebGestalt Liao et al.69 https://www.webgestalt.org GISTIC2.0 Mermel et al.70 ftp://ftp.broadinstitute.org/pub/ GISTIC2.0/GISTIC_2_0_23.tar.gz ragp Dragi cevi c et al.71 https://github.com/missuse/ragp protr Xiao et al.72 https://cran.r-project.org/web/ packages/protr/ NeoFlow Wen et al.37 https://github.com/bzhanglab/neoflow ComplexHeatmap Gu et al.73 https://bioconductor.org/packages/ release/bioc/html/ComplexHeatmap.html Optitype Szolek et al.74 github.com/FRED-2/OptiType NetMHCpan Jurtz et al.75 https://services.healthtech.dtu.dk/ service.php?NetMHCpan-4.0 customprodbj Wang et al.76 https://github.com/bzhanglab/ customprodbj PDV Li et al.77 https://github.com/wenbostar/PDV InteractiVenn Heberle et al.78 http://www.interactivenn.net/ ZigZag Thompson et al.79 https://github.com/ammonthompson/ zigzag KSEA Casado et al.80 and Wiredja et al.81 https://cran.r-project.org/web/ packages/KSEAapp/index.html Cytoscape v3.10.0 Shannon et al.82 https://cytoscape.org/ drc v3.0-1 Ritz et al.83 https://cran.r-project.org/web/ packages/drc ll OPEN ACCESS Please cite this article in press as: Savage et al., Pan-cancer proteogenomics expands the landscape of therapeutic targets, Cell (2024), https://doi.org/10.1016/j.cell.2024.05.039 Resource
Avidin-Biotin Assay:Article Title: Pan-cancer proteogenomics expands the landscape of therapeutic targets.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-integrin beta 5 Proteintech Cat# 28543-1-AP; RRID: AB_2881167 Rabbit monoclonal anti-CAD (clone D2T8H) Cell Signaling Technology Cat# 93925; RRID: AB_2750933 Rabbit anti-PAK2 Cell Signaling Technology Cat# 2608; RRID: AB_2283388 Mouse anti-GAPDH-HRP Yeasen Cat# 30203ES10 Mouse anti-IFN-g (clone 1-D1K) Mabtech Cat# 3420-3-1000; RRID: AB_907282 Mouse anti-IFN-g, biotin-conjugated (clone 7-B61) Mabtech Cat# 3420-6-1000; RRID: AB907272 Goat anti-rabbit IgG (H+L) HRP Yeasen Cat# 33101ES60; RRID: AB2922405 Biological samples PBMCs isolated from peripheral blood in healthy donors This paper Zhongshan Hospital, Fudan University PBMCs isolated from peripheral blood in healthy donors This paper Baylor College of Medicine Chemicals, peptides, and recombinant proteins Peptides China Peptides Inc. Genemed Synthesis Inc. N/A RPMI-1640 media cytiva Cat# SH30096.01 Click’s media IrvineScientific Cat# 9195 Human Ab serum Valley Biomedical, Inc. Cat# HP1022 Glutamax gibco Cat# 35050-061 Lymphoprep StemCell Technologies Cat# 07851 ImmunoCult-XF T cell expansion medium StemCell Technologies Cat# 10981 ImmunoCult Human CD3/CD28 T cell activator StemCell Technologies Cat# 10971 CD14 magnetic microbeads Miltenyi Biotec Cat# 130-050-201 CellGenix GMP DC media CellGenix Cat# 20801-0500 IL-1b R&D Systems Cat# 201-LB-025 IL-2 (Teceleukin) NCBI Cat# Ro 23-6019 IL-4 R&D Systems Cat# 204-IL/CF-MTO IL-6 R&D Systems Cat# 206-IL-050/CF IL-7 R&D Systems Cat# BT-007-01M IL-12 InvivoGen Cat# rcyc-hil12 IL-15 R&D Systems Cat# BT-015-01M GM-CSF R&D Systems Cat# 215-GM/CF-MTO TNF-ɑ R&D Systems Cat# 210TA100 PGE-2 Sigma Cat# P6532-1MG PHA-L Sigma-Aldrich Cat# L4144 Avidin-peroxidase solution Vector Laboratories Cat# PK-6100 AEC Sigma-Aldrich Cat# A6926 Tumor-associated antigens GenScript Biotech Corporation ImmunoCult Human CD3/CD28 T Cell Activator Stemcell Cat# 10971 Human recombinant IL-2 Stemcell Cat# 78036 Alvespimycin Selleckchem Cat# S1142 Tanespimycin Selleckchem Cat# S1141 Lipofectamine 3000 Thermo Cat# L3000015 Cell Counting Kit (CCK-8) Yeasen Cat# 40203ES80 (Continued on next page) e1 Cell 187, 1–19.e1–e10, August 8, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER NCI-H1944 Yongbo Wang lab (Department of Cellular and Genetic Medicine, School of Basic Medical Sciences, Fudan University) RRID: CVCL_1508 HT29 Dawei Li lab (Department of Colorectal Surgery, Fudan University Shanghai Cancer Center) RRID: CVCL_0320 Ishikawa QuiCell Biotechnology RRID: CVCL_2529 LOVO Daming Gao lab (Shanghai Institute of Biochemistry and Cell Biology) RRID: CVCL_0399 BXPC3 Yi Qin lab (Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center) RRID: CVCL_0186 Experimental models: Organisms/strains BALB/c Nude Shanghai Model Organisms Center Cat# SM-014 Oligonucleotides See Table S5B This study N/A Software and algorithms PepQuery2 Wen and Zhang12 http://www.pepquery.org metap Dewey68 https://cran.r-project.org/web/packages/ metap/index.html WebGestalt Liao et al.69 https://www.webgestalt.org GISTIC2.0 Mermel et al.70 ftp://ftp.broadinstitute.org/pub/ GISTIC2.0/GISTIC_2_0_23.tar.gz ragp Dragi cevi c et al.71 https://github.com/missuse/ragp protr Xiao et al.72 https://cran.r-project.org/web/ packages/protr/ NeoFlow Wen et al.37 https://github.com/bzhanglab/neoflow ComplexHeatmap Gu et al.73 https://bioconductor.org/packages/ release/bioc/html/ComplexHeatmap.html Optitype Szolek et al.74 github.com/FRED-2/OptiType NetMHCpan Jurtz et al.75 https://services.healthtech.dtu.dk/ service.php?NetMHCpan-4.0 customprodbj Wang et al.76 https://github.com/bzhanglab/ customprodbj PDV Li et al.77 https://github.com/wenbostar/PDV InteractiVenn Heberle et al.78 http://www.interactivenn.net/ ZigZag Thompson et al.79 https://github.com/ammonthompson/ zigzag KSEA Casado et al.80 and Wiredja et al.81 https://cran.r-project.org/web/ packages/KSEAapp/index.html Cytoscape v3.10.0 Shannon et al.82 https://cytoscape.org/ drc v3.0-1 Ritz et al.83 https://cran.r-project.org/web/ packages/drc ll OPEN ACCESS Please cite this article in press as: Savage et al., Pan-cancer proteogenomics expands the landscape of therapeutic targets, Cell (2024), https://doi.org/10.1016/j.cell.2024.05.039 Resource
Cell Counting:Article Title: Pan-cancer proteogenomics expands the landscape of therapeutic targets.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-integrin beta 5 Proteintech Cat# 28543-1-AP; RRID: AB_2881167 Rabbit monoclonal anti-CAD (clone D2T8H) Cell Signaling Technology Cat# 93925; RRID: AB_2750933 Rabbit anti-PAK2 Cell Signaling Technology Cat# 2608; RRID: AB_2283388 Mouse anti-GAPDH-HRP Yeasen Cat# 30203ES10 Mouse anti-IFN-g (clone 1-D1K) Mabtech Cat# 3420-3-1000; RRID: AB_907282 Mouse anti-IFN-g, biotin-conjugated (clone 7-B61) Mabtech Cat# 3420-6-1000; RRID: AB907272 Goat anti-rabbit IgG (H+L) HRP Yeasen Cat# 33101ES60; RRID: AB2922405 Biological samples PBMCs isolated from peripheral blood in healthy donors This paper Zhongshan Hospital, Fudan University PBMCs isolated from peripheral blood in healthy donors This paper Baylor College of Medicine Chemicals, peptides, and recombinant proteins Peptides China Peptides Inc. Genemed Synthesis Inc. N/A RPMI-1640 media cytiva Cat# SH30096.01 Click’s media IrvineScientific Cat# 9195 Human Ab serum Valley Biomedical, Inc. Cat# HP1022 Glutamax gibco Cat# 35050-061 Lymphoprep StemCell Technologies Cat# 07851 ImmunoCult-XF T cell expansion medium StemCell Technologies Cat# 10981 ImmunoCult Human CD3/CD28 T cell activator StemCell Technologies Cat# 10971 CD14 magnetic microbeads Miltenyi Biotec Cat# 130-050-201 CellGenix GMP DC media CellGenix Cat# 20801-0500 IL-1b R&D Systems Cat# 201-LB-025 IL-2 (Teceleukin) NCBI Cat# Ro 23-6019 IL-4 R&D Systems Cat# 204-IL/CF-MTO IL-6 R&D Systems Cat# 206-IL-050/CF IL-7 R&D Systems Cat# BT-007-01M IL-12 InvivoGen Cat# rcyc-hil12 IL-15 R&D Systems Cat# BT-015-01M GM-CSF R&D Systems Cat# 215-GM/CF-MTO TNF-ɑ R&D Systems Cat# 210TA100 PGE-2 Sigma Cat# P6532-1MG PHA-L Sigma-Aldrich Cat# L4144 Avidin-peroxidase solution Vector Laboratories Cat# PK-6100 AEC Sigma-Aldrich Cat# A6926 Tumor-associated antigens GenScript Biotech Corporation ImmunoCult Human CD3/CD28 T Cell Activator Stemcell Cat# 10971 Human recombinant IL-2 Stemcell Cat# 78036 Alvespimycin Selleckchem Cat# S1142 Tanespimycin Selleckchem Cat# S1141 Lipofectamine 3000 Thermo Cat# L3000015 Cell Counting Kit (CCK-8) Yeasen Cat# 40203ES80 (Continued on next page) e1 Cell 187, 1–19.e1–e10, August 8, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER NCI-H1944 Yongbo Wang lab (Department of Cellular and Genetic Medicine, School of Basic Medical Sciences, Fudan University) RRID: CVCL_1508 HT29 Dawei Li lab (Department of Colorectal Surgery, Fudan University Shanghai Cancer Center) RRID: CVCL_0320 Ishikawa QuiCell Biotechnology RRID: CVCL_2529 LOVO Daming Gao lab (Shanghai Institute of Biochemistry and Cell Biology) RRID: CVCL_0399 BXPC3 Yi Qin lab (Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center) RRID: CVCL_0186 Experimental models: Organisms/strains BALB/c Nude Shanghai Model Organisms Center Cat# SM-014 Oligonucleotides See Table S5B This study N/A Software and algorithms PepQuery2 Wen and Zhang12 http://www.pepquery.org metap Dewey68 https://cran.r-project.org/web/packages/ metap/index.html WebGestalt Liao et al.69 https://www.webgestalt.org GISTIC2.0 Mermel et al.70 ftp://ftp.broadinstitute.org/pub/ GISTIC2.0/GISTIC_2_0_23.tar.gz ragp Dragi cevi c et al.71 https://github.com/missuse/ragp protr Xiao et al.72 https://cran.r-project.org/web/ packages/protr/ NeoFlow Wen et al.37 https://github.com/bzhanglab/neoflow ComplexHeatmap Gu et al.73 https://bioconductor.org/packages/ release/bioc/html/ComplexHeatmap.html Optitype Szolek et al.74 github.com/FRED-2/OptiType NetMHCpan Jurtz et al.75 https://services.healthtech.dtu.dk/ service.php?NetMHCpan-4.0 customprodbj Wang et al.76 https://github.com/bzhanglab/ customprodbj PDV Li et al.77 https://github.com/wenbostar/PDV InteractiVenn Heberle et al.78 http://www.interactivenn.net/ ZigZag Thompson et al.79 https://github.com/ammonthompson/ zigzag KSEA Casado et al.80 and Wiredja et al.81 https://cran.r-project.org/web/ packages/KSEAapp/index.html Cytoscape v3.10.0 Shannon et al.82 https://cytoscape.org/ drc v3.0-1 Ritz et al.83 https://cran.r-project.org/web/ packages/drc ll OPEN ACCESS Please cite this article in press as: Savage et al., Pan-cancer proteogenomics expands the landscape of therapeutic targets, Cell (2024), https://doi.org/10.1016/j.cell.2024.05.039 Resource
|
R&D Systems is a verified supplier 
R&D Systems manufactures this product 